Based on an Innovative Principle
- Only correctly folded proteins are immobilized: Each protein is expressed with a small biotin carboxyl carrier protein (BCCP) tag, which binds to biotin only when the protein is properly folded. This ensures that functional proteins are immobilized on the streptavidin-coated array surface, optimizing assay performance by maintaining protein integrity and function.
- Misfolded proteins are washed away: If the protein is misfolded, the BCCP tag cannot bind to biotin, preventing immobilization and ensuring only correctly folded, functional proteins remain on the array.
- Immobilized proteins retain their native, functional structure: Each assay is conducted with full-length, correctly folded proteins, preserved on a non-denaturing surface to ensure accuracy and reliability.